| 质粒名称: | pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 107009 |
| 存储实验室: | Julian Downward |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CD274,PD-L1, B7-H1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1412 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XbaI（销毁后未知） |
| 3' 克隆位点: | BamHI（销毁后未知） |
| 5' 测序引物 | -- |
| 3' 测序引物 | pGL3-1: GAGCTGACTGGGTTGAAG |
|
文献引用方法 |
|
| 材料和方法部分: | pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final was a gift from Julian Downward (Addgene plasmid # 107009 ; http://n2t.net/addgene:107009 ; RRID:Addgene_107009) |
| 参考文献部分: | Oncogenic RAS Signaling Promotes Tumor Immunoresistance by Stabilizing PD-L1 mRNA. Coelho MA, de Carne Trecesson S, Rana S, Zecchin D, Moore C, Molina-Arcas M, East P, Spencer-Dene B, Nye E, Barnouin K, Snijders AP, Lai WS, Blackshear PJ, Downward J. Immunity. 2017 Dec 19;47(6):1083-1099.e6. doi: 10.1016/j.immuni.2017.11.016. Epub 2017 Dec 12. 10.1016/j.immuni.2017.11.016 PubMed 29246442 |
pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final 6166 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Modified Luciferase expression vector.
ACCESSION .
VERSION .
KEYWORDS pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6166)
AUTHORS Coelho MA, de Carne Trecesson S, Rana S, Zecchin D, Moore C,
Molina-Arcas M, East P, Spencer-Dene B, Nye E, Barnouin K, Snijders
AP, Lai WS, Blackshear PJ, Downward J
TITLE Oncogenic RAS Signaling Promotes Tumor Immunoresistance by
Stabilizing PD-L1 mRNA.
JOURNAL Immunity. 2017 Dec 19;47(6):1083-1099.e6. doi:
10.1016/j.immuni.2017.11.016. Epub 2017 Dec 12.
PUBMED 29246442
REFERENCE 2 (bases 1 to 6166)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6166
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 7..55
/note="synthetic polyadenylation signal"
misc_feature 69..160
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 109..128
/label=RVprimer3
/note="pGL3 vector, forward primer"
promoter 215..411
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 262..397
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 324..343
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
regulatory 441..450
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 447..2099
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(513..531)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
primer_bind complement(3665..3682)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3836..4424)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3916..3935)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4595..5455)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5218..5237
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5456..5560)
/gene="bla"
/label=AmpR promoter
rep_origin 5587..6042
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5674..5693)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5884..5905
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg
61 atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag
121 gctgtcccca gtgcaagtgc aggtgccaga acatttctct atcgataggt accgagctct
181 tacgcgtgct agcccgggct cgagatctgc gatctgcatc tcaattagtc agcaaccata
241 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
301 ccccatcgct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
361 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttggc
421 attccggtac tgttggtaaa gccaccatgg aagacgccaa aaacataaag aaaggcccgg
481 cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga
541 gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca
601 cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc
661 tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg
721 tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac
781 gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg
841 ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca
901 tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc
961 tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa
1021 ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc
1081 atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca
1141 ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta
1201 cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc
1261 tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat
1321 tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa
1381 ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc
1441 atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta
1501 cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga
1561 aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg
1621 tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga
1681 ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact
1741 tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg
1801 ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc
1861 ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga
1921 cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt
1981 tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg
2041 caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgtaat
2101 tctagagaaa aataacactg gaattccttt tctagcatta tatttattcc tgatttgcct
2161 ttgccatata atctaatgct tgtttatata gtgtctggta ttgtttaaca gttctgtctt
2221 ttctatttaa atgccactaa attttaaatt catacctttc catgattcaa aattcaaaag
2281 atcccatggg agatggttgg aaaatctcca cttcatcctc caagccattc aagtttcctt
2341 tccagaagca actgctactg cctttcattc atatgttctt ctaaagatag tctacatttg
2401 gaaatgtatg ttaaaagcac gtatttttaa aatttttttc ctaaatagta acacattgta
2461 tgtctgctgt gtactttgct atttttattt attttagtgt ttcttatata gcagatggaa
2521 tgaatttgaa gttcccaggg ctgaggatcc atgccttctt tgtttctaag ttatctttcc
2581 catagctttt cattatcttt catatgatcc agtatatgtt aaatatgtcc tacatataca
2641 tttagacaac caccatttgt taagtatttg ctctaggaca gagtttggat ttgtttatgt
2701 ttgctcaaaa ggagacccat gggctctcca gggtgcactg agtcaatcta gtcctaaaaa
2761 gcaatcttat tattaactct gtatgacaga atcatgtctg gaacttttgt tttctgcttt
2821 ctgtcaagta taaacttcac tttgatgctg tacttgcaaa atcacatttt ctttctggaa
2881 attccggcag tgtaccttga ctgctagcta ccctgtgcca gaaaagcctc attcgttgtg
2941 cttgaaccct tgaatgccac cagctgtcat cactacacag ccctcctaag aggcttcctg
3001 gaggtttcga gattcagatg ccctgggaga tcccagagtt tcctttccct cttggccata
3061 ttctggtgtc aatgacaagg agtaccttgg ctttgccaca tgtcaaggct gaagaaacag
3121 tgtctccaac agagctcctt gtgttatctg tttgtacatg tgcatttgta cagtaattgg
3181 tgtgacagtg ttctttgtgt gaattacagg caagaattgt ggctgagcaa ggcacatagt
3241 ctactcagtc tattcctaag tcctaactcc tccttgtggt gttggatttg taaggcactt
3301 tatccctttt gtctcatgtt tcatcgtaaa tggcataggc agagatgata cctaattctg
3361 catttgattg tcactttttg tacctgcatt aatttaataa aatattctta tttattttgt
3421 tacttggtac accagcatgt ccattttctt gtttattttg tgtttaataa aatgttcagt
3481 ttaacatccc agtggagaaa gttacttgga atatttgcgg atccgtcgac cgatgccctt
3541 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
3601 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg
3661 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
3721 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
3781 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
3841 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
3901 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
3961 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
4021 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
4081 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
4141 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
4201 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
4261 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
4321 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
4381 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
4441 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
4501 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
4561 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
4621 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
4681 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
4741 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
4801 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
4861 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
4921 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
4981 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
5041 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
5101 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
5161 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
5221 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
5281 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
5341 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
5401 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
5461 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
5521 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
5581 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
5641 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
5701 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
5761 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
5821 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
5881 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
5941 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
6001 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttgccattcg ccattcaggc
6061 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcccaagc
6121 taccatgata agtaagtaat attaaggtac gggaggtact tggagc
//
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